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Fig. 1. RT-PCR expression analysis of pig myosin isoforms (1, 2A, 2B, 2X) on different muscle tissue. (A) Samples were loaded onto agarose gel in the following order: masseter (M), longissimus dorsi (LD), semitendinosus red portion (Sr), diaphragm (D), white semitendinosus (Sw) and duplicated masseter (M'), longissimus dorsi (LD'), red semitendinosus (Sr'). s, standards; 1Kb Plus DNA Ladder (Invitrogen). The brightest band (500 bp) and bottom band (100 bp) of the molecular size markers are indicated. (B) The same isoforms were analysed in the retractor bulbi muscle (Rb). All MHC isoforms are expressed except for the slow isoform (type 1). The arrow indicates the position of the amplification of the slow isoform fragment (of 573 bp, see A) that is not expressed in this muscle. The band at 100 bp is due to primer aspecific anealing. s, markers as in A.





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