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Fig. 4. Effects of Na+-free medium or phloridzin (1 mmol l-1)
on 2-deoxyglucose (2-DG) uptake in cod (Gadus morhua) ventricle
strips. After dissection, strips were incubated for 60 min in either regular
medium (basal condition), Na+-free medium containing equimolar
choline, or regular medium containing 1 mmol l-1 phloridzin. All
media were supplemented with 5 mmol l-1 glucose, 35 mmol
l-1 mannitol and 0.1% bovine serum albumin (BSA). Ventricle strips
were rinsed in glucose-free medium and then assayed for glucose uptake
activity in the presence of 1 mmol l-1
2-deoxy-D-[3H(G)]glucose. Values are means ±
S.E.M. for six strips per bar. *P<0.01
versus basal condition.