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Fig. 5. Time courses of the transepithelial voltage normalized to the percentage of
the control value (46 mV and 22 mV, respectively), showing the
effects of ouabain (2.5 mmol l-1) during time period 1, of
BaCl2 (5 mmol l-1) during time period 2 and of amiloride
(0.2 mmol l-1) during time period 3. During the time period denoted
w, perfusion of the preparation was changed to withdrawal mode, establishing
the bathing solution also on the luminal side of the epithelium. Infusion mode
was used during the rest of the time.