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Fig. 4. Time course of the transepithelial voltage normalized to the percentage of
the control value (19 mV), showing the effects of ouabain and
concanamycin A. During the time period 1, ouabain (2.5 mmol l-1)
was present in the bath perfusate. During the time period 1+2, ouabain (2.5
mmol l-1) and concanamycin A (10 µmol l-1) were
present in the bath perfusate. Infusion mode was used throughout the
experiments shown. At the arrow, the preparation was withdrawn from the
perfusion pipette.