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Fig. 5. Immunocytochemical localization of the V-type H+-ATPase (A–D and G–I) and Na+/K+-ATPase (E,F). (A,C,E,G) Phase contrast and (B,D,F,H,I) fluorescence micrographs of 0.7 µm thick cryosections of the anterior sternal epithelium. (A,B) Control stage. (C–F) Stage of CaCO3 deposit formation. (G–I) Stage of CaCO3 deposit resorption. (I) Staining without amplification step. bc, blood cell; bl, basal lamina; cl, cuticle; n, nucleus. Scale bars, 25 µm (A–H); 16 µm (I).





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