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Fig. 7. Mean chloride cell (CC) number and size determined by LSC analysis from gill epithelial cell suspensions after acute or gradual acclimation from FW (blue diamonds) to 1x SW (green circles) or 2.4x SW (red triangles), respectively, versus acclimation period. (A) In the 1x SW group, CC volume is significantly larger at all the times analyzed (P<0.05) but does not continue to increase after 2 weeks. In contrast, during 5 weeks of 2.4x SW acclimation CC become significantly larger only after 2 weeks, but continue to increase (P<0.05). (B) CC number does not significantly increase until 2 weeks for 2.4x SW and until 5 weeks for 1x SW acclimation (P<0.05; aFW vs. 1x SW, bFW vs. 2.4x SW, c1x SW vs. 2.4x SW). N=4 animals for each salinity and time.





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