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Fig. 2. (A-C) Laser scanning cytometry (LSC) analysis of chloride cells (CC) from
killifish acclimated for 10 days to FW (blue), 1x SW (green) and
2.4x SW (red). CC were identified based on DASPMI fluorescence with the
LSC. CC area and maximal intensity of DASPMI fluorescence were recorded for
each CC during scanning by WinCyte software and plotted as scattergrams. (A)
FW; (B) 1x SW; (C) 2.4x SW. The boxes enclose all accepted data
and the circles enclose events that were used for generating image galleries
with the LSC relocation function. (D-F) Additional scattergrams showing the
relationship between CC area and integrated DASPMI fluorescence per CC were
also recorded. (D) FW; (E) 1x SW; (F) 2.4x SW. CCs have higher
DASPMI fluorescence than other cell types and are colored in all panels.