|
| |
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 4. Effect of cadmium alone and cadmium + Na+/H+
exchanger inhibitors on PK activity of isolated digestive gland cells of
Mytilus galloprovincialis. 100% represents enzyme activity in the
absence of cadmium (5.23±1.04 nmol min–1
mg–1 protein). Cells were treated for 30 min either with 50
µmol l–1 of cadmium alone, or 50 µmol
l–1 cadmium + 10 µmol l–1 amiloride (an
inhibitor of most plasma membrane Na+ transport systems) or + 20
nmol l–1 EIPA (a selective inhibitor of
Na+/H+ exchanger). PK activity in the presence of 20
nmol l–1 EIPA alone after 30 min of incubation was found to
be 5.38±0.7 nmol min–1 mg–1 protein.
The results (expressed as % PK activity of control value) are mean ±
S.D. of at least 7 independent experiments. In each
experiment, the tissue from four animals was used. *Significant
difference between the value after cadmium treatment and the control value;
significant difference between the values after cadmium
treatment with those observed after cadmium + inhibitors; Dunnet's test,
P<0.01.
|
