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Fig. 5. PCR amplification and cloning of the putative ßARK homologue in
Paramecium. (A) PCR product of 
400 bp (lane 2, arrowhead) was
electrophoretically transferred onto DEAE-cellulose. DNA was eluted and used
as a template for PCR reamplification resulting in the product of predicted
molecular size of 400 bp (B; lane 2, arrowhead). Following subcloning
into pGEM-T vector and transfection, the plasmid DNA was isolated from the
positive colonies (as described in Materials and methods) and subsequently
digested with EcoRI. The presence of an insert of the correct size
was detected (C; lane 2, arrowhead). Lanes 1 in A–C are molecular mass
standards.
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