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Fig. 3. (A–D) Dynamin-dependent sequestration of ß-adrenergic receptor
in isoproterenol-treated Paramecium cells, visualized by
post-embedding immunogold electron microscopy. Immunolocalization of ßAR
(10 nm gold particles) and dynamin (5 nm gold particles), was performed as
described in Materials and methods. Colocalization of ßAR and dynamin in
the small endocytic vesicles is observed (A, arrowheads; B–D). No or
only scarce ßAR on the surface membrane is observed (A, arrow). (E) In
untreated cells ßAR and dynamin colocalized on the plasma membrane.
(F–H) Immunolocalization of ßAR alone (F) and dynamin alone (G,H),
detected by anti-ßAR and anti-dynamin antibodies, respectively. A
significant presence of ßAR on the cell surface (F) occurs, whereas
dynamin was localized both on the membrane (H) and in the coated pits (G).
Bars, 100 nm.
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