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Fig. 2. Localization of dynamin and ß-adrenergic receptor (ßAR)
immunoanalogue in isoproterenol-treated and untreated Paramecium
cells using confocal laser scanning microscopy. Dual fluorochrome
immunolabeling was performed as described in Materials and methods.
Colocalization (yielding a yellow orange image) of ßAR (green) and
dynamin (red) inside the cell in small punctate accumulations (arrows) was
observed in the overlay of the series of confocal sections performed at 0.6
µm intervals (A–D). Such a pattern of colocalization was not observed
in the untreated cells (E). Bar, 16 µm.
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