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Fig. 1. Pictorial representation of the experimental design used to evaluate
effects of RU486 treatment in the Gambel's white-crowned sparrow. Spring
migrants and wintering birds were held on 16 h:8 h L:D and 8 h:16 h L:D,
respectively. (A) Experimental protocol designed to investigate the behavioral
responses of birds to RU486 treatment under ad libitum food
conditions. Treatment effects were tested by comparing birds one day before
and one day after RU486 injections. Controls received vehicle injections.
Birds were monitored for differences in locomotor activity (as determined by
motion detectors in registration cages) and daily food intake. (B)
Experimental protocol designed to investigate the behavioral and metabolic
responses of birds to RU486 treatment during a food manipulation trial. On the
morning following the second RU486 injection, food in chambers was removed for
four hours. Birds in a second chamber continued to experience ad
libitum food conditions. Locomotor activity and plasma metabolites were
monitored during the trial. After food was replaced, locomotor activity and
food intake were measured to determine how RU486 affects recovery. Controls
continued to receive vehicle injections.
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