Fig. 4. Minimum evolution phylogenetic tree of several vertebrate GS proteins. Drosophila mitochondrial and cytosolic GSase sequences were used as outgroups. Support for nodes (50% majority rule) is depicted on corresponding branches (ME bootstrap support/quartet-puzzling reliability values/Bayesian posterior probabilities are represented from left to right). Dashes indicate that there is no 50% majority rule support for the node under that analysis. Species are generally coded as in Murray et al. (2003), where the four-letter code refers to the first two letters of genus and species, followed by an accession number in either Genbank or SwissProt databases. Species are: Bosi, Bostrichthyes sinensis (sleeper); Onmy, Oncorhynchus mykiss (rainbow trout); Opbe, Opsanus beta (L,G, liver and gill) (gulf toadfish); Orni, Oreochromis niloticus (tilapia); SINFRUP, Takifugu rubripes (pufferfish); Dare, Danio rerio (zebrafish); Hefr, Heterodontus francisci (horned shark); Sqac, Squalus acanthias (spiny dogfish shark); Gaga, Gallus gallus (chicken); Hosa, Homo sapiens (human); Susc, Sus scrofa (pig); Acca, Acomys cahirinus (Egyptian spiny mouse); Crgr, Cricetulus griseus (Chinese hamster); Mumu, Mus musculus (mouse); Rano, Rattus norvegicus (rat); Xela, Xenopus laevis (clawed toad); Pali, Panulirus argus (spiny lobster); Paar, Paracentrotus lividus (sea urchin); Drme, Drosophila melanogaster (fruit fly). Scale bar represents proportion of amino acids per site.

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