Fig. 1. Western blots of several tissues from unconfined and confined representative individuals of the gulf toadfish Opsanus beta. The antibody is to a conserved oligopeptide sequence of GSases (homologous to amino acids 326-343 of the liver Gsase; Fig. 3). (A) Lanes 1 and 10 are molecular mass marker lanes. Even-numbered lanes are samples from unconfined toadfish and odd-numbered lanes from confined toadfish. Samples in lanes 2–3 are derived from brain; lanes 4–5, liver; lanes 6–7, kidney; lanes 8–9, stomach. (B) Lanes 1 and 9 are molecular mass marker lanes, and lane 10 is from unconfined brain (the same sample as in A, lane 2) for cross reference between gels. Even-numbered lanes are samples from unconfined toadfish and odd-numbered lanes from confined toadfish. Samples in lanes 2–3 are derived from intestine; lanes 4–5, gill; lanes 6–7, white muscle. Lane 8 is an empty well.

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