Fig. 6. Confocal images of myoinhibitory peptide-immunoreactivity (MIP-IR) in Manduca sexta adults. (A) Vibratome cross-section (200 µm) through the corpora cardiaca (CC) and aorta, showing immunoreactive endings near the surface of the CC. (B) Whole mount of the fifth abdominal ganglion of a pharate adult at stage-13 of development, showing strong labeling of the 704 interneurons (arrowhead), plus an anterior and posterior pair of interneurons (arrows), The latter are not labeled in the larval stages. (C) Whole mount of the fifth abdominal ganglion of a pharate adult shortly before eclosion, showing a pronounced reduction of MIP-immunostaining. (D) Whole mount of the fifth abdominal ganglion of a day-3 adult, showing an almost complete lack of staining. (E) Whole mount of the terminal abdominal ganglion of a stage-13 pharate adult, showing a reduction in MIP-immunostaining of the interneurons (arrowheads) and visceromotor neurons (arrows) (compare with Fig. 1D). (F) Whole mount of a terminal abdominal ganglion of a day-3 adult, showing an almost complete lack of MIPimmunostaining. (G) Epiproctodeal gland of a pharate adult at stage-8 of development, showing intense immunostaining of the neurohemal system on the wall of the rectum and proctodeal nerve (PN) and weak staining of the gland cells (arrowhead). (H) Whole mount of the epiproctodeal gland of a day-1 adult, showing diminished staining of the neurohemal system on the proctodeal nerves and on the wall of the rectum. At this stage, the immunostaining of the gland cells is too weak to be seen. Scale bars, 100 µm (A,H) and 200 µm (B–G).

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