Fig. 1. Confocal images of myoinhibitory peptide (MIP)-immunostained whole mounts of larval organs of Manduca sexta. (A) Brain of a thirdinstar larva, showing numerous small, MIP-immunoreactive neurons; the size and location of these cells indicate that they are not neurosecretory cells. (B) Corpus allatum (CA) and corpus cardiacum (CC) of an early fifth-instar larva, showing a lack of immunoreactive neurohemal processes. (C) Third abdominal ganglion of a third-instar larva, showing a pair of interneurons (704) and a lack of immunoreactive neurohemal processes in the perivisceral organ (PVO). (D) Terminal abdominal ganglion of a third-instar larva showing lateral cells that project into the eighth dorsal nerve (curved arrow) and a terminal cluster of mid-line neurons that project into the terminal nerve (straight arrow). (E) MIP-immunoreactive endings on muscles of the posterior region of the hindgut. Scale bars, 100 µm.

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