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Fig. 2. Method for determination of red blood cell concentration. (A) Vascular cast of a 4 d.p.f. zebrafish larva obtained by digital vascular contrasting method. (B) Edge detection using colour thresholding method. The frame in the tail indicates the region of interest (ROI). (C) Close up of the ROI. White circles indicate motion detected single blood cells. The vascular volume in the ROI is calculated from the geometric data of the vessel shown in B.





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