Fig. 3. PETH-immunoreactive Inka cells (stained orange/red) in pharate larval stages of hemimetabolous insects. In the cockroach Nauphoeta, tracheae on the surface of gonads contained Inka cells with narrow processes (A), while a different type of numerous single or coupled Inka cells lacking cytoplasmic processes were scattered throughout major broad tracheae (B). In the cricket Acheta, small Inka cells with few cytoplasmic processes were located on narrow tracheae (C), or more abundant larger cells with very prominent branching processes were found on the surface of broad tracheae (D). Numerous Inka cells with thick processes were distributed throughout the surface of narrow tracheae of the bugs Triatoma (E) and Pyrrhocoris (F,G). (H) PETH staining disappeared from all Inka cells of Pyrrhocoris after larval ecdysis. Scale bar, 50 µm in A—F; 100 µm in G,H.

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