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Fig. 10. Neuropeptide immunoreactivity in Inka cells of Bombyx before and after larval, pupal and adult ecdysis. Strong double staining with antibodies to small cardioactive peptide B (SCPB) and PBAN (pheromone biosynthesis activating neuropeptide; dark brown colour) in pharate 5th instar larvae (A) was depleted 5 min after ecdysis (B; arrow). PG, prothoracic gland. Strong reaction with the antiserum to FMRFamide in pharate pupae (C; blue colour) diminished at the onset of ecdysis (D). Intense staining with antibodies to SCPB and vasopressin (dark brown colour) in pharate adults (E) decreased considerably 5 min after eclosion (F). Scale bar, 200 µm.





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