Fig. 7. Cell function in response to UV application. Light was applied to the whole cilium, including both the basal body and upper region, at the anterior of a Paramecium cell loaded with NP-EGTA in Ca²⁺-containing medium. (A) A series of images around the UV application area (time course is indicated above each picture). UV light was applied to the area indicated as a circle in the picture marked 0.17 s. UV light was somewhat scattered by cytoplasmic particles. Arrowheads mark trichocysts released via exocytosis indicating the [Ca²⁺]_i at the basal bodies; arrows, direction of the cilia inside or outside the area of UV application. The series shows a typical result from a total of 13 experiments. Bar, 20 µm. This series of images are indicated in Movie 4, which is slowed to 1/3 of the original speed. (B) Temporal relationship of the ciliary angle measured from A. The time course in B matches the time indicated at the top of each picture in A. The period of UV application is indicated by the black bar; triangle, the time of trichocyst exocytosis; solid line, angle of a cilium within the UV application area; dashed line, angle of a cilium outside the area of UV application.

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