Fig. 4. Changes in [Ca²⁺]_i and cell functions of Paramecium cells loaded with NP-EGTA medium in response to UV light applied for 125 ms to the entire cell. [Ca²⁺]_i was estimated by monitoring the fluorescence intensity of Calcium Green in the Paramecium cell. (A) [Ca²⁺]_i values are expressed as PMT current. The value of PMT current was zero during UV application because the shutter in front of the PMT was closed to protect the PMT from the UV light. Arrow, UV application period. UV application caused an abrupt [Ca²⁺]_i in Paramecia loaded with NP-EGTA medium. A typical result from four experiments is shown. Ciliary reversal and trichocyst exocytosis in response to [Ca²⁺]_i is shown prior to (B) and following (C) UV application. Ciliary reversal took place all over the cell. Arrows, released trichocysts via exocytosis. Bar, 50 µm. A typical result from four experiments is shown.

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