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Fig. 3. Heterodimerization of MsGC-ß3 with MsGC-{alpha}1 and MsGC-ß1. Extracts of COS-7 cells that were transiently cotransfected with MsGC-ß3 and either empty vector (pcDNA3.1), or hexa-histidine (His6)-tagged MsGC-ß3{Delta}C338, MsGC-{alpha}1 or MsGC-ß1, were incubated with nickel-chelated agarose and the bound proteins analyzed by western blot. (A) Blot probed with MsGC-ß3 antiserum shows that MsGC-ß3 is pelleted when cotransfected with either tagged MsGC-ß3{Delta}C338, tagged MsGC-{alpha}1 or tagged MsGC-ß1, but the intensity of the immunoreactivity is highest when cotransfected with tagged MsGC-ß3{Delta}C338. (B) Western blot of input extract stained with anti-MsGC-ß3.





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