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Fig. 4. Motility-dependent dephosphorylation at serine residues in hypotonic conditions. Sperm were diluted in either 300 mmoll-1 NaCl solution (lane A), 50 mmoll-1 NaCl + 5 mmoll-1 CaCl2 (lane B), or 50 mmoll-1 NaCl + 5 mmoll-1 EGTA (lane C). Sperm were motile in the hypotonic solutions (containing 50 mmoll-1 NaCl; B,C) and immotile in the hypertonic solution (300 mmoll-1 NaCl; A). Sperm were collected and subjected to western blotting with anti-phosphoserine antibody. Numbers on the left indicate molecular mass markers. Motility is shown below the lanes.





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