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Fig. 3. Effect of (A) cAMP, cGMP and Ca2+, and (B) various concentrations of Ca2+ on demembranated sperm motility. Demembranated sperm were suspended in reactivation solutions containing 220 µmoll-1 Mg-ATP2+, 175 mmoll-1 potassium acetate, 1 mmoll-1 free Mg2+, 1 mmoll-1 DTT, 0.5 mmoll-1 EGTA, 0.5 mmoll-1 EDTA, 20 mmoll-1 Hepes-NaOH (pH 8.0). (A) Solutions contained 10 µmoll-1 cAMP, 10 µmoll-1 cGMP, 10-4 moll-1 free Ca2+ or reactivation solution only (ATP). (B) Effect of [Ca2+] on reactivation of sperm motility. Values are means ± S.D.; N=5 (A); N=7 (B).





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