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Fig. 4. itpr rescues the transport phenotype of the
itpr90B.0 allele. Fluid transport assays were performed on
the hsGAL4;itpr90B.0 line. The data show that
CAP2b-stimulated fluid transport is decreased in this line. Rescue
of hsGAL4;itpr90B.0 with UAS-itpr results in
wild-type levels of stimulated fluid transport. Fluid secretion rates were
measured for 30 min prior to addition of neuropeptide (arrow), after which
measurements were taken for a further 30 min. Data are expressed as mean fluid
secretion rates (nl min-1) ± S.E.M. (N=6-10).
UAS-itpr tubules display similar secretion rates to those of
wild-type tubules (data not shown).