Fig. 1. (A) Expression of PepT1-related mRNA in icefish and eel intestine by RT-PCR. The PepT1 isoform was detected in the intestine of icefish (lane 2) and eel (lane 4; positive control). The identity of each isoform was confirmed by PCR product size (570 bp) and sequencing (data not shown). Lanes 1 and 6: markers; lanes 3 and 5: RT-PCR control (RNA without reverse transcriptase). (B) Comparison of primary amino acid sequences of icefish and human PepT1 H⁺/peptide cotransporters. Asterisks indicate indentical amino acids; colons, conserved substitution (small, acidic, basal or hydroxyl+Amine+Basic, etc.); stops, semiconserved substitution.

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