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Fig. 2. The electrophysiological response of Paramecium to 25 µmol
l-1 ß,
-methylene ATP was a transient depolarization.
This is the ß,
-methylene ATP-receptor potential. As the response
was somewhat variable, two different cells are shown. The very fast spikes
seen in the first trace (Cell 1) are the Ca2+-based, graded action
potentials common to these cells but they are less evident in the second trace
(Cell 2). In both traces, the cells were exposed to 25 µmol l-1
ß,
-methylene ATP during the entire time. The recording medium
contained 1 mmol l-1 CaCl2, 1 mmol l-1 MOPS,
0.5 mmol l-1 MgCl2 and pH 7.2 with Tris-base. The
recording electrode contained 500 mmol l-1 KCl.