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Fig. 5. Sequences of footprinted regions observed in
Fig. 4. (A) Footprinting in the
termination region of tRNAleu. The conserved binding sequence of
mTERF (Valverde et al., 1994b)
is boxed. (B) The sequence in the control region located approximately 75 nt
5' of the tRNAmet gene in an area thought to contain the
L-strand promoter (Carrodeguas and Vallejo,
1997). The L strand was footprinted in both cases. Primers used
were LEU 1 for A and LSP 1 for B (see Table
1 for primers). Genomic positions are indicated. Relative to naked
DNA, overmethylated residues are filled squares and undermethylated residues
are unfilled squares.