Fig. 5. Na⁺ uptake in PNA⁺ mitochondria-rich gill cells of rainbow trout. Gill epithelial cells were collected from the 1.05-1.09 g ml^-1 Percoll density interface and further isolated using lectin binding and magnetic cell separation. Na⁺ influx rates were conducted as described in Materials and methods. Na⁺ influx rates were determined in the absence of any additional inhibitors (control; open histograms), 10 µmol l^-1 phenamil (Phen; shaded histograms), 10 nmol l^-1 bafilomycin A1 (Baf; hatched histograms) or a combination of phenamil and bafilomycin (Phen + Baf; filled histograms) and in either the absence (unstimulated) or presence (stimulated) of 10 mmol l^-1 Na⁺ proprionate. Data represent means + 1 S.E.M. (N=3). Means with different letters indicate that they are significantly different (P0.05).

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