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Fig. 3. Western blot analysis of eel swimbladder tissue with antibodies specific for vacuolar ATPase subunit B isoforms. 30 µg of swimbladder protein were used for each lane. Antibody #1034 recognizes both isoforms (rabbit anti-vatB1+vatB2), whereas antibody #1035 (rabbit anti-vatB1) is specific for subunit isoform vatB1. Both antibodies revealed bands of approximately 55 kDa. Without primary antibody, no bands could be detected.





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