Fig. 1. Na⁺/K⁺-ATPase (green) and Na⁺/K⁺/2Cl^- cotransporter (NKCC; red) immunoreactivity in gills of Hawaiian goby acclimated to freshwater (A–C) and 30 seawater (D–F). Double-staining was performed on the same sections, and the images combined in C and F. Images C and F were merged (from A,B and D,E, respectively) to highlight the colocalization of Na⁺/K⁺-ATPase and NKCC1 (red + green is yellow-orange). The width of each image is 207 µm.

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