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Fig. 2. SPaSE in males subjected to the four different conditions. (A-D) Percentage of males exhibiting auto-spermatophore extrusion (aSE; above) or the calling song (CS; below). (A) Free-moving condition. Note the very low percentage at 50-70 min of males exhibiting auto-spermatophore extrusion (aSE) compared to males exhibiting the calling song to a female (CS). (B) Restrained condition. (C) Semi-dissected condition. (D) Dissected condition. The columns at >120 min indicate the percentage of males that did not show auto-spermatophore extrusion within 120 min. Op. (C,D) indicates the period of the 10 min operation immediately after spermatophore preparation. M, median. These conventions are also used in Figs 3 and 10.





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