spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 4. Transient expression analysis of the rat myosin light chain (mylc) promoter in zebrafish embryos and structure of the mylc-GFP (A) and mylc-MSTNpro (B) transgenes. The mylc-GFP gene construct (A) contains a 1.3 kb rat myosin light chain promoter (mylc) at the 5', the 0.8 kb GFP coding region, a 0.8 kb SV40 poly(A) signal, and a mylc 1/3 enhancer from the rat mylc gene (Donoghue et al., 1991). The mylc-MSTNpro gene construct (B) contains the 1.3 kb rat myosin light chain promoter (mylc) at the 5', the 0.8 kb prodomain coding region of zebrafish myostatin (MSTNpro), the 0.8 kb SV40 polyA signal, and the mylc 1/3 enhancer from the rat mylc gene. The ATG start codon, RIRR proteolytic site and the TAA stop codon are indicated. (C,D) Muscle-specific expression of GFP reporter gene in 24 h.p.f. zebrafish embryos injected with the mylc-GFP: (C) fluorescence, (D) immunostaining. Scale bar, 100 µm. The mosaic pattern of expression is typical for transient expression studies in zebrafish embryos (Westerfield et al., 1992; Du and Dienhart, 2001).





Right arrow Return to article