Fig. 2. Expression of myostatin in zebrafish embryos, larvae, juvenile and adult. (A,B) In situ hybridization showing little or no myostatin expression in developing somites of zebrafish embryos at 24 h.p.f. (hours post-fertilization). (C) RT-PCR results showing myostatin (MSTN) expression in early stage zebrafish embryos, 1-4 d (days post-fertilization), swimming larvae (16 d), juvenile (1 month) and adult skeletal muscles (3 months). Elongation factor 1 (Ef-1) was used as control for RT-PCR. 16d, 1m and 3m are duplicated.

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