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Fig. 7. Recombinant strains of Bacillus thuringiensis subsp. israelensis (Bti) that produce the Bacillus sphaericus (Bs) 2362 binary toxin. (A) Acrystalliferous strain transformed with a plasmid that produces the Bs 2362 binary toxin using cyt1A promoters and the STAB/SD mRNA stabilizing sequence. (B) IPS-82 transformed with the same plasmid. A large crystal of the Bs binary toxin and a typical Bti crystal are obvious in the sporulated cell. (C) SDS-PAGE analysis of IPS-82 (lane Bti) and IPS-82 producing the Bti proteins and the Bs binary toxin (lane Bti/BsB).





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