Fig. 5. Southern blot analysis of a line derived from a germ-line transposition of a cn-carrying Mos1 vector. Line 16 was started from one individual whose parent was from line 128 and injected with a helper plasmid expressing before blastoderm formation. One of the resulting progeny had an eye color different from the parental insects and was used to establish line 16. Genomic DNA was isolated from adults and cut with SacI, which cuts twice within the gene vector, and transferred to a nylon membrane. The filter was hybridized with a radiolabeled cn+ gene fragment (see fig. 1 of Coates et al., 1998 for details of the analysis). An internal 2.5-kb fragment is present in lines 128 and 16. Additional hybridizing fragments are diagnostic of independent insertion sites within the genome. The difference in pattern between 128 and 16 indicates the presence of a transposition event.

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