Fig. 4. (A) Transposable element display analysis visualizing Hermes right-end junction fragments in individual Aedes aegypti containing the autonomous Hermes QH7010 element. c1 and c2 are controls: c1 is a non-transgenic wild-type mosquito and c2 is a transgenic mosquito containing a non-autonomous Hermes element also containing the actin5C:EGFP gene (Pinkerton et al., 2000). Bands in c1 and c2 are considered non-specific PCR products. Multiple, intensely labeled fragments were observed only from DNA prepared from the individuals containing the autonomous element. Molecular size markers, in base pairs, are shown. Bands isolated, reamplified and sequenced from this experiment are indicated (R2, R7, R10 and R11). (B) Labeled fragments were excised from transposable element display gels containing left- and right-hand Hermes ends (left-hand analysis not shown here), and their sequences were determined. Hermes inverted terminal repeat (ITR) sequences are indicated by the black arrows, and flanking A. aegypti sequences are shown with the proposed 8 bp target site duplications underlined. Only partial flanking sequences are shown, i.e. those immediately adjacent to the Hermes ITRs.

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