Fig. 5. Polyadenylation of mitochondrial mRNAs of A. franciscana embryos under control and various experimental treatments in organello. Following RACE-PAT, products were electrophoresed on 6% polyacrylamide at 100 V and stained with ethidium bromide. The fluorescence intensities for polyadenylated bands in each lane were quantified using ImageQuant software and normalized to the intensity of the upstream, non-adenylated band (uppermost band) and used in Figs 6 and 7. COXI (A), CYB (B), ND1 (C) and ATP6 (D); lane 1, control (30 min); lane 2, control (6 h); lane 3, low pH (30 min); lane 4, low pH (6 h); lane 5, low pH plus anoxia (1 h); lane 6, low pH plus anoxia (6 h); lane 7, anoxia (1 h); lane 8, anoxia (6 h); lane 9, ATP plus anoxia (0 h); lane 10, ATP plus anoxia (1 h); lane 11, ATP plus anoxia (6 h). M, markers (nucleotides). For CYB (B) and ND1 (C), lanes from two different gels are combined, and in all cases contrast has been adjusted among lanes to enhance visualization. For presentation purposes in this figure, the high intensity of the reference bands in B and D were uniformly reduced so the polyadenylated bands could be viewed at proper intensity.

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