Fig. 2. Alignment of cDNA sequences encoding part of the open reading frame (ORF) and 3' untranslated region (UTR) for fast and slow (S₁) MHCs to show the regions amplified by PCR. Bases that match one another are shaded, and the stop codon is enclosed in a box. The S₁ MHC sequence and the fast MHC sequence share 79% identity within the ORF. The 3' UTR follows the stop codon and has lower sequence identity. Fast forward (FF), fast reverse (FR), slow forward (SF) and slow reverse (SR) MHC primer positions are indicated by arrows adjacent to the fast and slow sequences, respectively. Reverse PCR primers were designed to anneal to specific sequences in the 3' UTR, while forward primers were designed to anneal to regions of relatively low sequence identity in the ORF. The fast MHC sequence has been published previously (Cotton and Mykles, 1993). The GenBank accession numbers for the fast and S₁ MHC sequences are U03091 and AY232598, respectively.

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