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Fig. 3. Uptake of [3H]L-leucine by KAAT1 wild-type (WT) and several mutants (Y147F, R76E and W75L). Accumulated radioactivity was measured in oocytes after 30 min exposure to Na+ media containing 0.2 mmol l-1 [3H]L-leucine. Oocytes had been injected 2-3 days earlier with cRNA encoding W75L, R76E, Y147F, WT or water control.





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