Fig. 1. Calanus helgolandicus embryos after TUNEL staining and observed by confocal laser scanning microscopy in fluorescence (A,C,E,G) and transmitted (B,D,F,H) light. (A) Fluorescent, three-dimensional image of an embryo produced by females fed for 10 days with the diatom Thalassiosira rotula. Nuclei are positively stained (green) by the TUNEL. Bar, 39.7 µm. (B) The same embryo as in A observed in transmitted light. Bar, 42.1 µm. (C) Fluorescent three-dimensional image of a C. helgolandicus embryo produced by female fed non-diatom Prorocentrum minimun (PRO) algae for 24 h then incubated for 1 h in 5 µg ml^-1 DD. Nuclei (green) are positively stained by TUNEL. Bar, 40 µm. (D) Embryo in C observed in transmitted light. (E) Three-dimensional image of the embryo produced by female fed 24 PRO. Nuclei are not stained in green and appear as black shadows. Bar, 42.2 µm. (F) The same embryo as in E observed in transmitted light. Bar, 40.3 µm. (G) Three-dimensional fluorescent image of a TUNEL-positive control embryo obtained by female fed for 24 h with PRO and incubated in DNase to simulate apoptosis. Nuclei are stained in green as in A and C. Bar, 34.6 µm. (H) The same embryo as in G observed in transmitted light. Bar, 34.6 µm.

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