Fig. 2. Retina of Manduca sexta. (A) Dorsal aspect of an unfixed retina dissected free of the cornea and clear zone. Anterior is to the right. The blue patch is the dorsal rim area. Scale bar, 0.5 mm. (B) Freehand vertical section of a glutaraldehyde-fixed retina photographed in reflected light. Dorsal is at the top, distal to the left. The highly reflective tracheoles (t) extend almost to the distal surface (ds) of the retina, except in the dorsal rim area (dr). Scale bar, 100 µm. (C) Retinulae dissected from the center of a fixed retina photographed in transmitted light: tracheoles are opaque, extending across the retina to just below the distal tips of the retinulae. bm, basement membrane beneath the retina. Scale bar, 10 µm. (D) Dorsal rim retinulae. Tracheoles envelop only the proximal ends of the retinulae. Scale bar, 10 µm.

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