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Fig. 4. Phylogenetic relationships of the S. domuncula RXR, RXR_SUBDO, with the related sequences from the coral Acropora millepora nuclear receptors TLL (TLL_ACROMI; AF323680), NR7 (NR7_ACROMI; AF323687) and NR8 (NR8_ACROMI; AF323688), Cnidaria Tripedalia cystophora retinoic acid X receptor (RXR_TRICYS; AF091121), Platyhelminthes Schistosoma mansoni retinoic acid receptor RXR (RXR_SCHMA; AF094759) and S. mansoni retinoid X receptor RXR-2 mRNA (RXR2_SCHMA; AF129816). In addition, relationships are shown with sequences from the Protostomia: D. melanogaster, seven-up protein (svp) type 1 (SVP_DROME; M28863), tailless (TLL_DROME; M34639), mRNA for XR2C ultraspiracle gene (USP_DROME; X53417), D. melanogaster nuclear receptor XR78E/F (XR78E_DROME; U31517); insects Locusta migratoria RXR mRNA (RXR_LOCMI; AF136372) and Tenebrio molitor THR6 (nuclear receptor) gene (TR2_TENMO; AJ005765); and from Deuterostomia: Danio rerio svp 46 mRNA for steroid receptor homologue (SVP46_DARE; X70300); human upstream promoter transcription factor (COUP-TF1_HUMAN; X58241), v-erbA related ear-2 gene (EAR2_HUMAN; X12794), the steroid hormone receptor hERR2 (ERR_HUMAN; X51417), hepatocyte nuclear factor 4 (HNF4_HUMAN; X76930), photoreceptor-specific nuclear receptor (PNR) (PNR_HUMAN; AF121129), receptor of retinoic acid-alpha (RAR{alpha}_HUMAN; X06614), retinoic acid receptor-like protein (RXR{alpha}_HUMAN; X52773), steroidogenic factor 1 mRNA (SF1_HUMAN; U76388), tailless gene homologue (TLX_HUMAN; Y13276) and the steroid receptor (TR2-11) (TR2_HUMAN; M29960); from Mus musculus germ cell nuclear factor (GCNF1_MOUSE; U14666) as well as the orphan steroid hormone receptor from Strongylocentrotus purpuratus (SHR2_STROPU; U38281). The different sequences were classified according to the unified proposition (NRNC, 1999). The tree was constructed as described in Materials and methods and remained unrooted. The numbers at the nodes indicate the level of confidence (%) for the branches as determined by bootstrap analysis (100 bootstrap replicates). The scale bar indicates an evolutionary distance of 0.1 aa substitutions per position in the sequence. Numbers in parentheses refer to the respective subfamilies/groups.





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