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Fig. 12. Phylogenetic relationships of the vertebrate MyoD sequences published to
date. Note that the cod sequence clusters with the seabream MyoD2 sequence.
Similarities are also evident in the in situ expression patterns. The
tree was constructed in PHYLIP
(Felsenstein 1995) by the
neighbour-joining method. Node numbers refer to the percentage of bootstrap
trials supporting a clade. Bootstrap confidence is based on 1000
pseudoreplications. The amino acid sequence for the single myogenic factor
(AMD1) of the ascidian Halocynthia roretzi (accession no. D13507) was
used as the outgroup. Other accession numbers are seabream Sparus
aurata MyoD1, AF478568, MyoD2 AF478569; blue tilapia Oreochromis
aureus, AF270790; Atlantic cod Gadus morhua, AF329903; pig
Sus scrofa, U12574; human Homo sapiens, NM_002478; sheep
Ovis aries, X62102; rat Rattus norvegicus, M84176; mouse
Mus musculus, XM_124916; chicken Gallus gallus, L34006;
Cotornix cotornix, L16686; Xenopus laevis xlmf1, M31116,
xlmf25 M31118; rainbow trout Oncorhynchus mykiss MyoD1,
X75798, MyoD2, Z46924; zebrafish Danio rerio, NM_131262; common carp
Cyprinus carpio, AB012882.
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