Fig. 2. Southern blots illustrating strong conservation of glycolytic enzyme gene sequences across species. Cells or tissues from the indicated organisms were lysed and genomic DNA was extracted by standard techniques (Webster, 1987; Webster et al., 1990; Lonberg and Gilbert, 1985). DNA was digested with restriction enzyme EcoRI, separated on agarose gels and blotted onto nitrocellulose. Membranes were probed with ³²P-cDNAs coding for pyruvate kinase (PK) and lactate dehydrogenase (LDH) as described in Webster (1987). Arrows indicate conserved DNA fragments. Note the increased number of hybridization bands for both genes in rodents (blocks indicated by vertical bars) that probably represent increased numbers of pseudogenes in these species (see text).

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