Fig. 7. Rescue of tax-4 defect by cell-specific expression. The tax-4(p678) worms expressing the tax-4 cDNA::gfp gene under indicated promoters were examined for their behavior. (A) Population distribution was determined with worms carrying the tax-4 expression construct and kin-8::gfp as an injection marker. Control worms were injected solely with kin-8::gfp. Combined results of 2-3 experiments are shown. Total worm numbers analysed and experiment numbers (in parentheses) were as follows: control; 74 (3), 49 (2), 80 (3); tax-4p, 76 (3), 71 (3), 54 (2); nhr-38p, 74 (3), 69 (3), 78 (3); gcy-8p, 56 (2), 45 (2), 39 (2); gpa-3p, 63 (3), 80 (3), 82 (3). (B,C) Movement was examined with worms transformed by a tax-4 cDNA expression construct without an injection marker. Control represents the results of the parent tax-4(p678). (B) Means of instantaneous velocities (open symbols) and their elements in gradient directions (closed symbols; positive values indicate upward movement; negative values indicate downwards movement) are shown. Total numbers of analysed intervals of each line were as follows: no transgene, 1109; tax-4p, 249 and 448; nhr-38p, 439, 494 and 303; gcy-8p, 271 and 754; gpa-3p, 575 and 518. In both distribution and movement analysis, two or three lines were tested for each transformation and are shown with different symbols. (C) Examples of traces.

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