Fig. 3. Representative western immunoblots showing the time course of phosphorylation of endogenous eIF2 with HCR (heme controlled repressor kinase). Post-mitochondrial supernatant was isolated from H. aspersa hepatopancreas (A) or from N. sutor liver (B) and incubated with HCR in the presence of 40 µmol l^-1 ATP. Aliquots were removed immediately prior to the addition of HCR (0 min) and at 20 min, 40 min and 60 min after the addition of HCR. Proteins were separated using SDS–PAGE, transferred to nitrocellulose membranes then probed with anti-eIF2(P).

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