Fig. 5. (A) Western blot of Rimicaris exoculata abdomen samples for the detection of hsp70 proteins (anti-human hsp70). Each lane corresponds to a different individual. The `reference' lanes show individuals from Expt 2 (24 h; 15°C); the `heat-shock' lanes show individuals from Expt 4 (24 h; 25°C heat shock; see Materials and methods). Arrowheads indicate the hsp70 proteins, named band HMM (high molecular mass) and LMM (low molecular mass). (B) Comparison of hsp70 levels in abdomen samples as estimated by density measurements of the western blot protein bands (HMM and LMM). R = reference samples, HS = heat-shocked samples. Density profiles were obtained from the western blot membranes using NIH Image 1.6 software. The density of each band (expressed in arbitrary units) was calculated from the area of the corresponding peak on the profile. Each column represents the mean of band density for four individuals (± S.D.). The asterisk indicates a significant difference between treatments (Mann–Whitney test, U=0.0, P=0.021).

Return to article