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Fig. 8. Effect of different free Ca2+ concentrations on parameters of ADP kinetics of mitochondrial respiration in permeabilized myocardial fibers and in myosin-extracted (ghost) fibers. (A) Effect of Ca2+ on apparent Km for ADP. A dramatic decline in the apparent Km for ADP was observed in control fibers. By contrast, no changes in apparent Km for ADP can be seen in ghost fibers. (B) Effect of different free Ca2+ concentrations on Vmax.





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