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Fig. 7. Imaging of mitochondria in permeabilized myocardial fibers after extraction of myosin (ghost fibers) by membrane-potential-sensitive probe teramethylrhodamine ethyl ether (TMRE). (A) Ghost fibers in the presence of 2mmoll-1 ATP, 2mmoll-1 malate and 5mmoll-1 glutamate (concentration of free Ca2+ in Ca-EGTA buffer: 0.1 µmoll-1). (B) The same ghost fibers after addition of calcium chloride (final concentration of free Ca2+ in Ca-EGTA buffer: 1.0 µmoll-1). No structural changes were seen. The same result was obtained for a free Ca2+ concentration of 3 µmoll-1.





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